Troponin I Type 3 (cardiac) Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33463

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Troponin I/TNNI3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunocytochemistry/ Immunofluorescence: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunocytochemistry/Immunofluorescence: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human cell line HEK 293 shows localization to cytosol & vesicles.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human prostate shows low expression as expected.
Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-33463] - Staining in human heart muscle and prostate tissues using anti-TNNI3 antibody. Corresponding TNNI3 RNA-seq data are presented for the same tissues.

Applications for Troponin I Type 3 (cardiac) Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Equally reactive with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. (In the presence of 5 mM EDTA). Antibodies are not affected by heparin, phosphorylation, oxidation and troponin complex formation. No cross-reaction with skeletal muscle troponin I.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Gene Symbol

TNNI3

UniProt

P19429

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Troponin I Type 3 (cardiac) Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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