TRPC1 Antibody

Novus Biologicals | Catalog # NBP2-62000

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRPC1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NLKQKRDENYQK, from the internal region of the protein sequence according to NP_003295.1; NP_001238774.1.

Epitope

C-NLKQKRDENYQK

Reactivity Notes

Preditced to cross react with Mouse, Canine and Rat

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat TRPC1 Antibody (NBP2-62000) is a polyclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRPC1 Antibody

Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NBP2-62000]

Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NBP2-62000]

Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic/Vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).
Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000]

Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000]

Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human prostate. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: TRPC1 Antibody [NBP2-62000]

Flow Cytometry: TRPC1 Antibody [NBP2-62000]

Flow Cytometry: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NBP2-62000]

Immunocytochemistry/ Immunofluorescence: TRPC1 Antibody [NBP2-62000]

Immunocytochemistry/Immunofluorescence: TRPC1 Antibody [NBP2-62000] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml).
Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000]

Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000]

Immunohistochemistry-Paraffin: TRPC1 Antibody [NBP2-62000] - Paraffin embedded human cortex. Antibody at 3.75 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TRPC1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection Limit 1:32000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPC1

The TRP1 (TRPC1) gene encodes for a short transient receptor potential channel 1 protein which creates non-selective channels permeable to calcium and other cations. The long isoform is 793 amino acids long at around 91 kDA while the short isoform is 759 amino acids at approximately 87 kDA. These proteins are believed to be operated by a phosphatidylinositol messenger system. The TRP1 gene interacts with MX1, CAV1, ITPR3, HOMER1, and BMPR2 genes in pathways for CREB transcription, intracellular calcium signaling, developmental biology and pancreatic secretion. The TRP1 gene has been studied in various diseases such as kidney disease, breast cancer, neuroblastoma, prostatius, retinitis, basal cell carcinoma, muscular dystrophy, gingivitis, hypoxia, cerebritis, neurodegeneration, pyloric stenosis, and bipolar disorder.

Long Name

Short transient receptor potential channel 1

Alternate Names

TRP-1, TRP1

Gene Symbol

TRPC1

Additional TRPC1 Products

Product Documents for TRPC1 Antibody

Certificate of Analysis

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Product Specific Notices for TRPC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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