TRPM7 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-20224

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all TRPM7 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the 4th cytoplasmic loop of human TRPM7 conjugated to an immunogenic carrier protein was used as the immunogen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRPM7 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody - Azide and BSA Free [NBP1-20224]

Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody - Azide and BSA Free [NBP1-20224]

Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG, DAPI counterstained.
Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody - Azide and BSA Free [NBP1-20224]

Immunocytochemistry/ Immunofluorescence: TRPM7 Antibody - Azide and BSA Free [NBP1-20224]

Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - IF on PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG, DAPI counterstained appearing in blue.

Applications for TRPM7 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

30 ug/ml

Immunohistochemistry

10-50 ug/ml

Immunohistochemistry-Paraffin

10-50 ug/ml

Western Blot

10-50 ug/ml

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRPM7

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.; FUNCTION: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).; CATALYTIC ACTIVITY: ATP + a protein: ADP + a phosphoprotein.; COFACTOR: Binds 1 zinc ion per subunit.; SUBUNIT: Homodimer. Forms heterodimers with TRPM6.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

Alternate Names

CHAK, CHAK1FLJ25718, Channel-kinase 1, EC 2.7.11.1, Long transient receptor potential channel 7, LTRPC ion channel family member 7, LTrpC7, LTrpC-7, LTRPC7FLJ20117, transient receptor potential cation channel subfamily M member 7, transient receptor potential cation channel, subfamily M, member 7, transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK

Entrez Gene IDs

54822 (Human)

Gene Symbol

TRPM7

UniProt

Q96QT4 (Mouse)

Additional TRPM7 Products

Product Documents for TRPM7 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRPM7 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRPM7 Antibody - Azide and BSA Free

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Protocols

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