TRPV4 Antibody

Novus Biologicals | Catalog # NB110-74960

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TRPV4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 300-400 of mouse TRPV4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Reactivity Notes

Marmoset (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPV4 Antibody

Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960]

Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960]

Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer).
Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960]

Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960]

Immunohistochemistry-Paraffin: TRPV4 Antibody [NB110-74960] - Rat kidney using Rabbit antibody to TRPV4, dilution 1: 1000, incubated 30 min at RT (using Autostainer)

Applications for TRPV4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:1000-1:2000

Immunohistochemistry-Paraffin

1: 1000 - 1: 2000

Western Blot

1:1000-1:2000
Application Notes
ICC/IF, IHC reactivity reported in (PMID: 22761937).

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRPV4

TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permanent cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

Long Name

Transient receptor potential cation channel subfamily V member 4

Alternate Names

HMSN2C, OTRPC4, SSQTL1, TRP12, VR-OAC, VRL-2, VRL2, VROAC

Entrez Gene IDs

59341 (Human); 63873 (Mouse); 66026 (Rat)

Gene Symbol

TRPV4

UniProt

Q9EPK8 (Mouse)

Additional TRPV4 Products

Product Documents for TRPV4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TRPV4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TRPV4 Antibody

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  • Q: We have a customer who is looking for an antibody that is suitable to detect TRPV4/Vanilloid receptor-like protein 2 in sheep FFPE tissues. Could you allign the immunizing peptid of NB110-74960 and NB100-98910 against the sheep sequence (if you have it - I could not find the sheep TRPV4 sequence in UniProt and also, the customer did not supply the sequence) and inform us on the homology between the peptids used to generate NB110-74960/NB100-98910 and the sheep sequence?

    A: Sheep and Human TRPV4 are over 90% similar, so most antibodies to the human sequence will be predicted to cross-react. NB110-74960 is probably the best choice for this.

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