Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRPV4 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid peptide near the center of human TRPV4. The immunogen is located within amino acids 380 - 430 of TRPV4. Amino Acid Squence: RREVTDEDTRHLSRKFKD

Specificity

TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRPV4 Antibody - BSA Free

Western Blot: TRPV4 AntibodyBSA Free [NBP2-41262]

Western Blot: TRPV4 AntibodyBSA Free [NBP2-41262]

Western Blot: TRPV4 Antibody [NBP2-41262] - Analysis of TRPV4 in (A) human testis tissue and (B) SK-N-SH cell lysate with TRPV4 antibody at 1 ug/mL.
TRPV4 Antibody - BSA Free

Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] -

Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human lung tissue with TRPV4 antibody at 5 ug/mL.
TRPV4 Antibody - BSA Free

Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] -

Immunohistochemistry: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunohistochemistry of TRPV4 in human kidney tissue with TRPV4 antibody at 2 ug/ml.
TRPV4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] -

Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 ug/mL.
TRPV4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] -

Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] - Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 ug/ml.

Red: TRPV4 Antibody
Blue: DAPI staining
TRPV4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRPV4 Antibody - BSA Free [NBP2-41262] -

Immunofluorescence for Vim (red) and TRP or Piezo channels (green) in cultured LP-ICs. Double staining reveals the protein expression of TRPV4 (A), piezo1 (B), TRPA1 (C), TRPV2 (D), TRPV1 (E), TRPM8 (F) and Piezo2 (G) in most of the Vim+ LP-ICs. The nucleus marker (DAPI) is stained in blue. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35069237), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TRPV4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPV4

TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permanent cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.

Long Name

Transient receptor potential cation channel subfamily V member 4

Alternate Names

HMSN2C, OTRPC4, SSQTL1, TRP12, VR-OAC, VRL-2, VRL2, VROAC

Gene Symbol

TRPV4

Additional TRPV4 Products

Product Documents for TRPV4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRPV4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRPV4 Antibody - BSA Free

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Protocols

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