TRPV5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94490

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRPV5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 655-725 of human TRPV5 (NP_062815.3). DKEDDQEHPSEKQPSGAESGTLARASLALPTSSLSRTASQSSSHRGWEILRQNTLGHLNLGLNLSEGDGEE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRPV5 Antibody - BSA Free

TRPV5 Antibody - BSA Free

Western Blot: TRPV5 Antibody - BSA Free [NBP2-94490] -

Western Blot: TRPV5 Antibody - BSA Free [NBP2-94490] - Western blot analysis of various lysates, using TRPV5 Rabbit pAb (A6473) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
TRPV5 Antibody - BSA Free

Immunohistochemistry: TRPV5 Antibody - BSA Free [NBP2-94490] -

Immunohistochemistry: TRPV5 Antibody - BSA Free [NBP2-94490] - Immunohistochemistry analysis of paraffin-embedded human kidney using TRPV5 Rabbit pAb (A6473) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Applications for TRPV5 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRPV5

TRPV5 is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. [provided by RefSeq]

Long Name

Transient Receptor Potential Cation Channel Subfamily V Member 5

Alternate Names

CAT2, ECAC1, OTRPC3

Gene Symbol

TRPV5

Additional TRPV5 Products

Product Documents for TRPV5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRPV5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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