TRPV6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-74138

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Synthetic peptides corresponding to the middle region of Trpv6. Immunizing peptide sequence TEIDSSGDDQSLLELIVTTKKREARQILDQTPVKELVSLKWKRYGRPYFC. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for TRPV6 Antibody - BSA Free

Western Blot: TRPV6 Antibody [NBP1-74138]

Western Blot: TRPV6 Antibody [NBP1-74138]

Western Blot: TRPV6 Antibody [NBP1-74138] - Sample Type: hRetinal pigment epithelial cells, 4 individual donors (20ug) Primary Dilution: 1:1000 Secondary Antibody: goat anti-rabbit-AP Secondary Dilution: 1:2000 Image Submitted by: Brian Kennedy Indiana University Northwest
Western Blot: TRPV6 Antibody [NBP1-74138]

Western Blot: TRPV6 Antibody [NBP1-74138]

Western Blot: TRPV6 Antibody [NBP1-74138] - Rat Brain Lysate 1ug/ml Gel Concentration 12%

Applications for TRPV6 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRPV6

Trpv6 is a Ca(2+)-sensing Ca(2+) channel.

Long Name

Transient Receptor Potential Cation Channel Subfamily V Member 6

Alternate Names

ABP/ZF, CaT-L, CaT1, ECAC2, ZFAB

Gene Symbol

TRPV6

UniProt

Additional TRPV6 Products

Product Documents for TRPV6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TRPV6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TRPV6 Antibody - BSA Free

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  • Q: What are the recommended dilutions for Western blot?

    A: I have found that the following conditions have been successfully used with this antibody (generated a good image): Primary dilution: 1:1000, Secondary dilution: 1:2000 of goat anti-rabbit alkaline phosphatase conjugate. This would be a good starting point to try, but we suggest that you optimize the conditions for your particular experiment.

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