Tryptophan Hydroxylase Pan Specific Antibody

R&D Systems | Catalog # PPS038

R&D Systems
Discontinued Product
PPS038 has been discontinued. View all Tryptophan Hydroxylase products.

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Tryptophan Hydroxylase Primary Antibodies »

Product Specifications

Immunogen

Tryptophan Hydroxylase

Specificity

This antibody is specific for the ~55 kDa TPH in Western blots of human dorsal Raphe nucleus lysates. Immunolabeling has been demonstrated in rat brain lysates. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. Due to antigen modifications in E. coli, this antibody does not recognize TPH in rabbit tissues.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Tryptophan Hydroxylase Pan Specific Antibody

Detection of Tryptophan Hydroxylase antibody by Western Blot.

Detection of Tryptophan Hydroxylase by Western Blot.

Western blot of human dorsal Raphe nucleus. As shown in the autoradiograph, the antibody is specific for the ~55 kDa TPH protein.

Applications for Tryptophan Hydroxylase Pan Specific Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 dilution

Western Blot

1:1000 dilution
Sample: Human brain (dorsal Raphe nucleus)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: Tryptophan Hydroxylase

Tryptophan hydroxylase (TPH) is a 51 kDa, 444 amino acid (aa) enzyme that belongs to the pterin-dependent, aromatic amino-acid hydroxylase family. It is found in peripheral tissues and pineal tissues, and catalyzes the rate-limiting step in serotonin generation from L‑tryptophan. The molecule contains two domains, an N-terminal regulatory domain (aa 1-110) and a C-terminal catalytic domain (aa 111-444) that contains an iron atom. TPH is constitutively active but unstable. Phosphorylation by PKA at S58 stabilizes the enzyme and increases its activity by 25%. This action is complemented by 14-3-3 proteins that physically interact with phosphorylated TPH to increase both its stability and activity by another 15% to 45% of baseline. Binding of the acidic carboxyterminus of 14-3-3 with the regulatory region of TPH also blocks phosphatase activity directed towards TPH, prolonging its activity. One splice variant of TPH is known. It occurs at the extreme C‑terminus where there is a 29 aa substitution for the last seven amino acids. This may generate a more stable enzyme.

References

  1. Boularand, S. et al. (1990) Nucleic Acids Res. 18:4257.
  2. Wang, G-A. et al. (1998) J. Neurochem. 71:1769.
  3. Kumer, S.C. et al. (1997) J. Neurochem. 69:1738.
  4. Wang, L. et al. (2002) Biochemistry 41:12569.
  5. Banik, U. et al. (1997) J. Biol. Chem. 272:26219.
  6. Haycock, J.W. et al. (2002) J. Neurosci. Methods. 114:205.

Alternate Names

TPH, TPH1

Product Documents for Tryptophan Hydroxylase Pan Specific Antibody

Certificate of Analysis

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Product Specific Notices for Tryptophan Hydroxylase Pan Specific Antibody

For research use only

Citations for Tryptophan Hydroxylase Pan Specific Antibody

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Protocols

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