Tryptophanyl tRNA synthetase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20744

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Tryptophanyl tRNA synthetase Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Tryptophanyl tRNA synthetase

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tryptophanyl tRNA synthetase Antibody - BSA Free

Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744]

Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744]

Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744]

Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744]

Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - U87 xenograft, using Tryptophanyl tRNA synthetase antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744]

Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744]

Immunohistochemistry-Paraffin: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - Mouse prostate. Tryptophanyl tRNA synthetase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Tryptophanyl tRNA synthetase Antibody

Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] -

Western Blot: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tryptophanyl tRNA synthetase antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Tryptophanyl tRNA synthetase Antibody - BSA Free

Immunohistochemistry: Tryptophanyl tRNA synthetase Antibody [NBP2-20744] -

Tryptophanyl tRNA synthetase antibody detects Tryptophanyl tRNA synthetase protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Tryptophanyl tRNA synthetase antibody (NBP2-20744) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for Tryptophanyl tRNA synthetase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tryptophanyl tRNA synthetase

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

EC 6.1.1, EC 6.1.1.2, GAMMA-2, hWRS, IFI53, IFP53trpRS, Interferon-induced protein 53, TrpRS, tryptophan tRNA ligase 1, cytoplasmic, Tryptophan--tRNA ligase, tryptophanyl-tRNA synthetase, tryptophanyl-tRNA synthetase, cytoplasmic, WRS

Gene Symbol

WARS1

UniProt

P23381 (Human)

Additional Tryptophanyl tRNA synthetase Products

Product Documents for Tryptophanyl tRNA synthetase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Tryptophanyl tRNA synthetase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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