TSC2 Antibody (SC05-59)

Novus Biologicals | Catalog # NBP2-67552

Recombinant Monoclonal Antibody.
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SC05-59 expressed in HEK293
View all TSC2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human TSC2 aa 1760-1807 / 1807. (SwissProt: P49815 Human; SwissProt: Q61037 Mouse; SwissProt: P49816 Rat)

Localization

Cytoplasm, Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit TSC2 Antibody (SC05-59) (NBP2-67552) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TSC2 Antibody (SC05-59)

Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552]

Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552]

Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Western blot analysis of TSC2 on MCF-7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab
Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552]

Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552]

Immunohistochemistry-Paraffin: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of paraffin-embedded mouse placenta tissue using anti-Tuberin antibody. Counter stained with hematoxylin.
Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552]

Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552]

Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Flow cytometric analysis of TSC2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).
Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552]

Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552]

Western Blot: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Tuberin on Jurkat cell lysates using anti-Tuberin antibody at 1/1,000 dilution.
Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552]

Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552]

Flow Cytometry: TSC2 Antibody (SC05-59) [NBP2-67552] - Analysis of Hela cells with Tuberin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Applications for TSC2 Antibody (SC05-59)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSC2

Tuberous sclerosis complex-2 (TCS2 also known as Tuberin) is a tumor suppressor that forms a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Additionally, binding of 14-3-3 beta to TSC2 at Ser1210 reduce the ability of the complex to inhibit phosphorylation of S6 kinase (4). Phosphorylation by ATK at Ser924 and Thr1518 inactivates TSC2 and disrupts its interaction with TSC1 (5). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Gene Symbol

TSC2

Additional TSC2 Products

Product Documents for TSC2 Antibody (SC05-59)

Certificate of Analysis

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Product Specific Notices for TSC2 Antibody (SC05-59)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

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Protocols

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