TSFM Antibody

Novus Biologicals | Catalog # NBP2-20746

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TSFM Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TSFM. The exact sequence is proprietary.

Reactivity Notes

Mouse (89%), Rat (88%).

Localization

Mitochondrion

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TSFM Antibody

Western Blot: TSFM Antibody [NBP2-20746]

Western Blot: TSFM Antibody [NBP2-20746]

Western Blot: TSFM Antibody [NBP2-20746] - Sample (30 ug of whole cell lysate) A: Jurkat B: K562 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: TSFM Antibody [NBP2-20746]

Immunohistochemistry-Paraffin: TSFM Antibody [NBP2-20746]

Immunohistochemistry-Paraffin: TSFM Antibody [NBP2-20746] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.
TSFM Antibody

Immunocytochemistry/ Immunofluorescence: TSFM Antibody [NBP2-20746] -

TSFM antibody detects TSFM protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: TSFM protein stained by TSFM antibody (NBP2-20746) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for TSFM Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TSFM

Synthesis of the 13 mitochondrial-encoded proteins occurs on a dedicated mitochondrial translation apparatus similar to that found in prokaryotes and requires, in addition to the tRNAs and rRNAs encoded in mtDNA, the concerted action of several translation factors and a large number of mitochondrial ribosomal proteins, all of which are encoded by nuclear genes. The TSFM gene encodes a mitochondrial translation elongation factor (Smeitink et al., 2006 [PubMed 17033963]).[supplied by OMIM]

Alternate Names

COXPD3, EFTS, EF-Ts, EFTSMT, EF-TsMt, mitochondrial, mitochondrial elongation factor Ts, Ts translation elongation factor, mitochondrial

Gene Symbol

TSFM

Additional TSFM Products

Product Documents for TSFM Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TSFM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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