TSPAN7/TM4SF2 Antibody (CL0262) - BSA Free
Novus Biologicals | Catalog # NBP2-52894
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0262
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P41732, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TSPAN7/TM4SF2 Antibody (CL0262) - BSA Free
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] -
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] - Analysis in human cerebral cortex and testis tissues using NBP2-52894 antibody. Corresponding TSPAN7 RNA-seq data are presented for the same tissues.![Western Blot: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894]](https://resources.rndsystems.com/images/products/TSPAN7-TM4SF2-Antibody-CL0262-Western-Blot-NBP2-52894-img0004.jpg "Western Blot: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894]")
Western Blot: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894]
Western Blot: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: TSPAN7 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] -
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] - Staining of human cerebral cortex shows moderate positivity in neuropil.
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] -
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] - Staining of human cerebellum shows moderate positivity in neuropil.
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] -
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] -
Immunohistochemistry-Paraffin: TSPAN7/TM4SF2 Antibody (CL0262) [NBP2-52894] -Staining of human testis shows no positivity in cells in seminiferous ducts as expected.Applications for TSPAN7/TM4SF2 Antibody (CL0262) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TSPAN7
Long Name
Tetraspanin 7
Alternate Names
A15, CCG-B7, CD231, DXS1692E, MRX58, MXS1, TALLA-1, TM4SF2, TM4SF2b
Gene Symbol
TSPAN7
Additional TSPAN7 Products
Product Documents for TSPAN7/TM4SF2 Antibody (CL0262) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TSPAN7/TM4SF2 Antibody (CL0262) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for TSPAN7/TM4SF2 Antibody (CL0262) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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