TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44302

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL14523

Format

Azide and BSA Free
View all TTF-1/NKX2-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P43699, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free

TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Analysis in human thyroid gland and skeletal muscle tissues using NBP3-44302 antibody. Corresponding TTF-1 / NKX2-1 RNA-seq data are presented for the same tissues.
TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Staining of human liver shows no positivity in hepatocytes as expected.
TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Staining of human lung shows strong nuclear positivity in a subset of pneumocytes.
TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Staining of human thyroid gland shows strong nuclear positivity in glandular cells.
TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free Western Blot: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Western Blot: TTF-1 / NKX2-1 Antibody [NBP3-44302]

Analysis in human cell line SCLC-21H.

Applications for TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TTF-1/NKX2-1

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Gene Symbol

NKX2-1

Additional TTF-1/NKX2-1 Products

Product Documents for TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TTF-1 / NKX2-1 Antibody (CL14523) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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