TTF-1 / NKX2-1 Antibody (CL14531) - BSA Free

Novus Biologicals | Catalog # NBP3-26862

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL14531

Format

BSA Free
View all TTF-1/NKX2-1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P43699, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TTF-1 / NKX2-1 Antibody (CL14531) - BSA Free

TTF-1 / NKX2-1 Antibody (CL14531)

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (CL14531) [NBP3-26862]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
TTF-1 / NKX2-1 Antibody (CL14531)

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (CL14531) [NBP3-26862]

Analysis in human lung and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
TTF-1 / NKX2-1 Antibody (CL14531)

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (CL14531) [NBP3-26862]

Staining of human liver shows no positivity in hepatocytes as expected.
TTF-1 / NKX2-1 Antibody (CL14531)

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (CL14531) [NBP3-26862]

Staining of human lung shows strong nuclear positivity in a subset of pneumocytes.
TTF-1 / NKX2-1 Antibody (CL14531)

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (CL14531) [NBP3-26862]

Staining of human thyroid gland shows strong nuclear positivity in glandular cells.
TTF-1 / NKX2-1 Antibody (CL14531)

Western Blot: TTF-1 / NKX2-1 Antibody (CL14531) [NBP3-26862]

Western blot analysis in human cell line SCLC-21H.

Applications for TTF-1 / NKX2-1 Antibody (CL14531) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TTF-1/NKX2-1

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Long Name

NK2 Homeobox 1

Alternate Names

BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1

Gene Symbol

NKX2-1

Additional TTF-1/NKX2-1 Products

Product Documents for TTF-1 / NKX2-1 Antibody (CL14531) - BSA Free

Certificate of Analysis

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Product Specific Notices for TTF-1 / NKX2-1 Antibody (CL14531) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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