Tubby Antibody

Novus Biologicals | Catalog # NBP2-20758

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Tubby Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tubby. The exact sequence is proprietary.

Reactivity Notes

Bovine (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tubby Antibody

Western Blot: Tubby Antibody [NBP2-20758]

Western Blot: Tubby Antibody [NBP2-20758]

Western Blot: Tubby Antibody [NBP2-20758] - Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758]

Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758]

Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758] - Mouse fore brain. Tub antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Tubby Antibody [NBP2-20758]

Western Blot: Tubby Antibody [NBP2-20758]

Western Blot: Tubby Antibody [NBP2-20758] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758]

Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758]

Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758] - Cal27 xenograft, using Tub antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758]

Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758]

Immunohistochemistry-Paraffin: Tubby Antibody [NBP2-20758] - Rat fore brain. Tub antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Tubby Antibody

Immunocytochemistry/ Immunofluorescence: Tubby Antibody [NBP2-20758] -

Tub antibody detects Tub protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tub protein stained by Tub antibody (NBP2-20758) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Tubby Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tubby

This gene encodes a member of the Tubby family of bipartite transcription factors. The encoded protein may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, and it functions as a membrane-bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Tubby Bipartite Transcription Factor

Alternate Names

RD5, RDOB, Tubby Homolog

Gene Symbol

TUB

Additional Tubby Products

Product Documents for Tubby Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tubby Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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