Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43837

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Drosophila, Fish, Goat, Zebrafish

Predicted:

Bovine (100%), Chimpanzee (99%), Porcine (100%), Rabbit (100%), Rhesus Macaque (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 114

Format

Azide and BSA Free
View all Tubulin alpha-1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Paraffin-embedded mouse colon. Red: alpha Tubulin antibody [114] diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (NBP1-32239) diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug NIH-3T3 whole cell lysate/extract C. 30 ug mouse brain lysate/extract D. 30 ug PC-12 whole cell lysate/extract E. 30 ug rat brain lysate/extract F. 30 ug drosophila lysae/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:5000.
Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of A. 30 ug zebrafish eye lysate/extract 10 % SDS-PAGE alpha Tubulin antibody [114] dilution: 1:1000.
Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody at a dilution of 1:10000.
Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human cervix. alpha Tubulin antibody [114] diluted at 1:500.
Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody (114) [NBP2-43837] - Analysis of human lung cancer. alpha Tubulin antibody [114] diluted at 1:500.
Tumorsphere: Tubulin alpha-1B Antibody (114) [NBP2-43837]

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Antibody (114) diluted at 1:10000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Immunohistochemistry-Frozen: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

Immunofluorescence photomicrographs of frozen sections of mouse brain.
Green: alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488).
Blue: Nuclear staining with Hoechst 33342.
Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Western Blot: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:250.
Red: NeuN, stained by NeuN antibody diluted at 1:250.
Blue: Fluoroshield with DAPI.
Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (114) [NBP2-43837] -

TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20C 100% methanol for 5 min.
Green: TUBA1B protein stained by TUBA1B antibody (NBP2-43837) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:20000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 20% Glycerol

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tubulin alpha-1B

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain

Long Name

Tubulin alpha-1B chain

Alternate Names

TUBA1B, Tubulin K-alpha-1

Gene Symbol

TUBA1B

Additional Tubulin alpha-1B Products

Product Documents for Tubulin alpha-1B Antibody (114) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Tubulin alpha-1B Antibody (114) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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