Tubulin alpha-1B Antibody (ARC51243)
Novus Biologicals | Catalog # NBP3-23538
Recombinant Monoclonal Antibody
Loading...
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # ARC51243
Loading...
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-42 of human alpha Tubulin(NP_006073.2, P68363).
Epitope
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTI
Localization
cytoplasm, cytoplasmic microtubule, microtubule cytoskeleton
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Tubulin alpha-1B Antibody (ARC51243)
Western Blot: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Western Blot: Tubulin alpha-1B Antibody [NBP3-23538] - Western blot analysis of lysates from HeLa cells, using Tubulin alpha-1B Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.5s.
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] - Confocal imaging of HeLa cells using alpha -Tubulin Rabbit mAb(Green). The cells were counterstained with RAB11A Rabbit mAb. DAPI was used for nuclear staining (blue). Objective: 60x.
Immunohistochemistry: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Immunohistochemistry: Tubulin alpha-1B Antibody [NBP3-23538] - Immunohistochemistry analysis of Tubulin alpha-1B in paraffin-embedded rat colon tissue using Tubulin alpha-1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Immunohistochemistry: Tubulin alpha-1B Antibody [NBP3-23538] - Immunohistochemistry analysis of Tubulin alpha-1B in paraffin-embedded human cervix cancer tissue using Tubulin alpha-1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Immunohistochemistry: Tubulin alpha-1B Antibody [NBP3-23538] - Immunohistochemistry analysis of Tubulin alpha-1B in paraffin-embedded mouse testis tissue using Tubulin alpha-1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Immunohistochemistry: Tubulin alpha-1B Antibody [NBP3-23538] - Immunohistochemistry analysis of Tubulin alpha-1B in paraffin-embedded human colon carcinoma tissue using Tubulin alpha-1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Immunohistochemistry: Tubulin alpha-1B Antibody [NBP3-23538] - Immunohistochemistry analysis of Tubulin alpha-1B in paraffin-embedded human tonsil tissue using Tubulin alpha-1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry: Tubulin alpha-1B Antibody (ARC51243) [NBP3-23538] -
Immunohistochemistry: Tubulin alpha-1B Antibody [NBP3-23538] - Immunohistochemistry analysis of Tubulin alpha-1B in paraffin-embedded mouse kidney tissue using Tubulin alpha-1B Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for Tubulin alpha-1B Antibody (ARC51243)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Tubulin alpha-1B
Long Name
Tubulin alpha-1B chain
Alternate Names
TUBA1B, Tubulin K-alpha-1
Gene Symbol
TUBA1B
Additional Tubulin alpha-1B Products
Product Documents for Tubulin alpha-1B Antibody (ARC51243)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Tubulin alpha-1B Antibody (ARC51243)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for Tubulin alpha-1B Antibody (ARC51243)
There are currently no reviews for this product. Be the first to review Tubulin alpha-1B Antibody (ARC51243) and earn rewards!
Have you used Tubulin alpha-1B Antibody (ARC51243)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...