Tubulin alpha-1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20760

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Drosophila, Zebrafish

Predicted:

Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Tubulin alpha-1B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Tubulin alpha-1B. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (100%).

Marker

Microtubule Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tubulin alpha-1B Antibody - BSA Free

Western Blot: Tubulin alpha-1B Antibody [NBP2-20760]

Western Blot: Tubulin alpha-1B Antibody [NBP2-20760]

Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 10% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20760]

Immunocytochemistry/ Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20760]

Immunocytochemistry/Immunofluorescence: Tubulin alpha-1B Antibody [NBP2-20760] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TUBA1B protein stained by alpha Tubulin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20760]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20760]

Immunohistochemistry-Paraffin: Tubulin alpha-1B Antibody [NBP2-20760] - Human colon carcinoma, using alpha Tubulin antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Tubulin alpha-1B Antibody

Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20760] -

Immunohistochemistry: Tubulin alpha-1B Antibody [NBP2-20760] - Tubulin alpha-1B antibody detects Tubulin alpha-1B protein on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tubulin alpha-1B antibody (NBP2-20760) dilution: 1:200.
Tubulin alpha-1B Antibody

Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] -

Western Blot: Tubulin alpha-1B Antibody [NBP2-20760] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B antibody (NBP2-20760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for Tubulin alpha-1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Tubulin alpha-1B

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Long Name

Tubulin alpha-1B chain

Alternate Names

TUBA1B, Tubulin K-alpha-1

Gene Symbol

TUBA1B

Additional Tubulin alpha-1B Products

Product Documents for Tubulin alpha-1B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Tubulin alpha-1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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