TUFM Antibody (900)

Novus Biologicals | Catalog # NBP3-11261

Novus Biologicals
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Key Product Details

Species Reactivity

E. coli

Applications

Western Blot, Immunoassay, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 900
View all TUFM Primary Antibodies »

Product Specifications

Immunogen

E. coli K-12 C600 Rif ( pKT146) bacteria

Epitope

The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for TUFM Antibody (900)

Application
Recommended Usage

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TUFM

The monoclonal antibody 900 recognizes elongation factor Tu (EF-Tu), a 43 kDa multifunctional protein present in Escherichia coli. EF-Tu is one of the most abundant proteins present in prokaryotes, representing about 5 % of the total cellular protein of E. coli. During protein biosynthesis, the elongation process, EF-Tu catalyzes the binding of each aminoacyl-tRNA to the ribosome. It also interacts with several macromolecules and guanine nucleotides, including EF-Ts, GDP, GTP, and some ribosomal proteins. Monoclonal antibody 900 recognizes EF-Tu in organisms belonging to bacterial and archaeal domains, yet no organisms from the eukaryotic domain. The panbacterial distribution of EF-Tu, which is present in large amounts in every prokaryotic cell, renders this protein a good candidate for diagnostic purposes. The highly conserved epitope recognized by monoclonal antibody 900 is located at the very end of the N-terminus of the EF-Tu molecule (SKEKFE).

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

Additional TUFM Products

Product Documents for TUFM Antibody (900)

Certificate of Analysis

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Product Specific Notices for TUFM Antibody (900)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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