TUFM Antibody (CL2243) - BSA Free

Novus Biologicals | Catalog # NBP2-36752

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL2243

Format

BSA Free
View all TUFM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P49411, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TUFM Antibody (CL2243) - BSA Free

Western Blot: TUFM Antibody (CL2243) [NBP2-36752]

Western Blot: TUFM Antibody (CL2243) [NBP2-36752]

Western Blot: TUFM Antibody (CL2243) [NBP2-36752] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts, as well as in Leydig cells.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in HeLa cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human cell line A431 showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in MCF7 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2243) [NBP2-36752] - Staining in U2OS cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human fallopian tube shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2243) [NBP2-36752] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules and moderate positivity in glomeruli.

Applications for TUFM Antibody (CL2243) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TUFM

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

UniProt

P49411 (Human)

Additional TUFM Products

Product Documents for TUFM Antibody (CL2243) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for TUFM Antibody (CL2243) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TUFM Antibody (CL2243) - BSA Free

There are currently no reviews for this product. Be the first to review TUFM Antibody (CL2243) - BSA Free and earn rewards!

Have you used TUFM Antibody (CL2243) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies