TUFM Antibody (CL2245) - BSA Free

Novus Biologicals | Catalog # NBP2-36753

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2245

Format

BSA Free
View all TUFM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P49411, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TUFM Antibody (CL2245) - BSA Free

Western Blot: TUFM Antibody (CL2245) [NBP2-36753]

Western Blot: TUFM Antibody (CL2245) [NBP2-36753]

Western Blot: TUFM Antibody (CL2245) [NBP2-36753] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753] - Staining in U251 cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular epithelium cells.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753] - Staining in HeLa cell line showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753] - Staining of human cell line A431 showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753] - Staining in MCF7 cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/ Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753]

Immunocytochemistry/Immunofluorescence: TUFM Antibody (CL2245) [NBP2-36753] - Staining in U2OS cell line with Anti-TUFM monoclonal antibody, showing distinct mitochondrial staining in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753] - Staining of human skin shows granular cytoplasmic immunoreactivity in epithelial cells.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753] - Staining of human placenta shows strong cytoplasmic positivity in trophoblast.
Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753]

Immunohistochemistry-Paraffin: TUFM Antibody (CL2245) [NBP2-36753] - Staining of human kidney shows granular cytoplasmic immunoreactivity in renal tubules.

Applications for TUFM Antibody (CL2245) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TUFM

TUFM encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq]

Alternate Names

EFTu, EF-Tu, EFTUCOXPD4, EF-TuMT, elongation factor Tu, mitochondrial, P43, Tu translation elongation factor, mitochondrial

Gene Symbol

TUFM

UniProt

P49411 (Human)

Additional TUFM Products

Product Documents for TUFM Antibody (CL2245) - BSA Free

Certificate of Analysis

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Product Specific Notices for TUFM Antibody (CL2245) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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