Tumstatin/COL4A3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-02967

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Tumstatin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1555-1669 of human Tumstatin/COL4A3 (NP_000082.2). AIAIAVHSQTTDIPPCPHGWISLWKGFSFIMFTSAGSEGTGQALASPGSCLEEFRASPFLECHGRGTCNYYSNSYSFWLASLNPERMFRKPIPSTVKAGELEKIISRCQVCMKKR

Specificity

Possible crossreactivity to whole COL4A3 molecule.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Tumstatin/COL4A3 Antibody - BSA Free

Western Blot: Tumstatin/COL4A3 AntibodyAzide and BSA Free [NBP3-02967]

Western Blot: Tumstatin/COL4A3 AntibodyAzide and BSA Free [NBP3-02967]

Western Blot: Tumstatin/COL4A3 Antibody [NBP3-02967] - Western blot analysis of extracts of Mouse kidney, using Tumstatin/COL4A3 antibody (NBP3-02967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Tumstatin/COL4A3 Antibody

Immunocytochemistry/Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] -

Immunocytochemistry/Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] - Analysis of NIH/3T3 cells using Tumstatin/COL4A3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Tumstatin/COL4A3 Antibody

Immunocytochemistry/Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] -

Immunocytochemistry/Immunofluorescence: Tumstatin/COL4A3 Antibody [NBP3-02967] - Analysis of HeLa cells using Tumstatin/COL4A3 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Tumstatin/COL4A3 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Tumstatin/COL4A3 Antibody - Azide and BSA Free [NBP3-02967] -

Immunocytochemistry/ Immunofluorescence: Tumstatin/COL4A3 Antibody - Azide and BSA Free [NBP3-02967] - Immunofluorescence analysis of PC-12 cells using Tumstatin/COL4A3 Rabbit pAb (A16359) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Tumstatin/COL4A3 Antibody - BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Tumstatin

COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity.

Alternate Names

COL4A3

Gene Symbol

COL4A3

Additional Tumstatin Products

Product Documents for Tumstatin/COL4A3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Tumstatin/COL4A3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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