Two pore calcium channel protein 2 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP1-30670
![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]](https://resources.rndsystems.com/images/products/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0005.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
A synthetic peptide from amino acid region 700-752 of human Two pore calcium channel protein 2 conjugated to blue carrier protein was used as the antigen.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Two pore calcium channel protein 2 Antibody - Azide and BSA Free
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human gallbladder. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]](https://resources.rndsystems.com/images/products/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0001.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]")
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]](https://resources.rndsystems.com/images/products/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0002.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]")
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]](https://resources.rndsystems.com/images/products/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0003.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]")
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.![Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]](https://resources.rndsystems.com/images/products/Two-pore-calcium-channel-protein-2-Antibody-Immunohistochemistry-Paraffin-NBP1-30670-img0004.jpg "Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]")
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody - Azide and BSA Free [NBP1-30670]
Immunohistochemistry-Paraffin: Two pore calcium channel protein 2 Antibody [NBP1-30670] - Sections of human lung. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin. Primary antibody: dilution 10 ug/ml, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.Applications for Two pore calcium channel protein 2 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
10-50 ug/ml
Immunohistochemistry-Paraffin
10-50 ug/ml
Western Blot
10-50 ug/ml
Application Notes
For IHC-Paraffin: Antigen retrieval is essential.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Reconstitution
Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.
Formulation
Sterilized water
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: Two pore calcium channel protein 2
Long Name
Two pore channel protein 2
Alternate Names
TPC2, TPCN2
Entrez Gene IDs
233979 (Mouse)
Gene Symbol
TPCN2
UniProt
Additional Two pore calcium channel protein 2 Products
Product Documents for Two pore calcium channel protein 2 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Two pore calcium channel protein 2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Two pore calcium channel protein 2 Antibody - Azide and BSA Free
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Q: When doing a Western Blot, what is the expected MW of the band that I should detect with this antibody?
A: In Western Blot application, two pore calcium channel protein 2 Antibody (NBP1-30670) is expected to detect a band at around 85kD MW.
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