TXNIP Antibody (JM60-35)

Novus Biologicals | Catalog # NBP2-75692

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM60-35 expressed in HEK293
View all TXNIP Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human TXNIP aa 50-99 / 391. (SwissProt: Q9H3M7 Human; SwissProt: Q8BG60 Mouse; SwissProt: Q5M7W1 Rat)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TXNIP Antibody (JM60-35)

Western Blot: TXNIP Antibody (JM60-35) [NBP2-75692]

Western Blot: TXNIP Antibody (JM60-35) [NBP2-75692]

Western Blot: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of TXNIP on different lysates using anti-TXNIP antibody at 1/1,000 dilution.Positive control: Lane 1: PC-12 Lane 2: Human skeletal muscleLane 3: NIH-3T3 Lane 4: Hela
Immunocytochemistry/ Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunocytochemistry/ Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow Cytometry: TXNIP Antibody (JM60-35) [NBP2-75692]

Flow Cytometry: TXNIP Antibody (JM60-35) [NBP2-75692]

Flow Cytometry: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of Hela cells with TXNIP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Immunocytochemistry/ Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunocytochemistry/ Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunocytochemistry/ Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunocytochemistry/Immunofluorescence: TXNIP Antibody (JM60-35) [NBP2-75692] - Staining TXNIP in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human kidney tissue using anti-TXNIP antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human stomach cancer tissue using anti-TXNIP antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse kidney tissue using anti-TXNIP antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse stomach tissue using anti-TXNIP antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded human stomach tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692]

Immunohistochemistry-Paraffin: TXNIP Antibody (JM60-35) [NBP2-75692] - Analysis of paraffin-embedded mouse esophagus tissue with Rabbit anti-TXNIP antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for TXNIP Antibody (JM60-35)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:400

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TXNIP

TXNIP may act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppressionof CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nu

Alternate Names

HHCPA78, THIF, thioredoxin binding protein 2, thioredoxin interacting protein, Thioredoxin-binding protein 2, thioredoxin-interacting protein, upregulated by 1,25-dihydroxyvitamin D-3, VDUP1EST01027, Vitamin D3 up-regulated protein 1

Gene Symbol

TXNIP

Additional TXNIP Products

Product Documents for TXNIP Antibody (JM60-35)

Certificate of Analysis

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Product Specific Notices for TXNIP Antibody (JM60-35)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TXNIP Antibody (JM60-35)

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Protocols

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