TYRP1 Antibody (CL4923) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44070

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4923

Format

Azide and BSA Free
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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P17643, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TYRP1 Antibody (CL4923) - Azide and BSA Free

TYRP1 Antibody (CL4923) - Azide and BSA Free Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP3-44070]

Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP3-44070]

Staining of human tonsil shows no positivity in lymphoid cells as expected (negative control).
TYRP1 Antibody (CL4923) - Azide and BSA Free Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP3-44070]

Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP3-44070]

Staining of human skin shows strong cytoplasmic immunoreactivity in melanocytes.
TYRP1 Antibody (CL4923) - Azide and BSA Free Western Blot: TYRP1 Antibody [NBP3-44070]

Western Blot: TYRP1 Antibody [NBP3-44070]

Analysis in human cell line SK-MEL-30.
TYRP1 Antibody (CL4923) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TYRP1 Antibody [NBP3-44070]

Immunocytochemistry/ Immunofluorescence: TYRP1 Antibody [NBP3-44070]

Staining of SK-MEL-30 cells using the Anti-TYRP1 monoclonal antibody).

Applications for TYRP1 Antibody (CL4923) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TYRP1

TYRP1 encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq]

Alternate Names

b-PROTEIN, CAS25,6-dihydroxyindole-2-carboxylic acid oxidase, Catalase B, CATB, EC 1.14.18, EC 1.14.18.-, EC 1.14.18.1, Glycoprotein 75, GP75DHICA oxidase, Melanoma antigen gp75, TRP-1, TRPTYRP, tyrosinase-related protein 1TRP1, TYRRP

Gene Symbol

TYRP1

Additional TYRP1 Products

Product Documents for TYRP1 Antibody (CL4923) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TYRP1 Antibody (CL4923) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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