U2AF2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33397

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western, Knockdown Validated

Cited:

RNA immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all U2AF2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for U2AF2 Antibody - BSA Free

Western Blot: U2AF2 Antibody [NBP2-33397]

Western Blot: U2AF2 Antibody [NBP2-33397]

Western Blot: U2AF2 Antibody [NBP2-33397] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.
Immunohistochemistry: U2AF2 Antibody [NBP2-33397]

Immunohistochemistry: U2AF2 Antibody [NBP2-33397]

Immunohistochemistry: U2AF2 Antibody [NBP2-33397] - Staining of human colon shows strong nuclear positivity in glandular cells.
Simple Western: U2AF2 Antibody [NBP2-33397]

Simple Western: U2AF2 Antibody [NBP2-33397]

Simple Western: U2AF2 Antibody [NBP2-33397] - Simple Western lane view shows a specific band for U2AF2 in 0.2 mg/ml of HEK 293T lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: U2AF2 Antibody [NBP2-33397]

Simple Western: U2AF2 Antibody [NBP2-33397]

Simple Western: U2AF2 Antibody [NBP2-33397] - Electropherogram image of the corresponding Simple Western lane view. U2AF2 antibody was used at 1:25 dilution on HEK 293T lysate(s) respectively.

Applications for U2AF2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
See Simple Western Antibody Database for Simple Western validation: Tested in HEK 293T lysate, separated by Size, antibody dilution of 1:25, apparent MW was 61 kDa

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: U2AF2

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date. [provided by RefSeq]

Alternate Names

hU2AF(65), hU2AF65, splicing factor U2AF 65 kD subunit, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 small nuclear RNA auxiliary factor 2, U2AF65U2 snRNP auxiliary factor large subunit

Gene Symbol

U2AF2

UniProt

P26368 (Human)

Additional U2AF2 Products

Product Documents for U2AF2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for U2AF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for U2AF2 Antibody - BSA Free

Customer Reviews for U2AF2 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review U2AF2 Antibody - BSA Free and earn rewards!

Have you used U2AF2 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies