UbcH7/UBE2L3 Antibody (24GB1875)

Novus Biologicals | Catalog # NBP3-51593

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB1875
View all UbcH7/UBE2L3 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human UbcH7/UBE2L3 (Uniprot: P68036)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UbcH7/UBE2L3 Antibody (24GB1875)

UbcH7/UBE2L3 Antibody (24GB1875)

Immunocytochemistry/ Immunofluorescence: UbcH7/UBE2L3 Antibody (24GB1875)[NBP3-51593] -

Immunocytochemical staining of HepG2 cells with UbcH7/UBE2L3 antibody (1:1,000). Nuclei were stained blue with DAPI; UbcH7/UBE2L3 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
UbcH7/UBE2L3 Antibody (24GB1875)

Flow Cytometry: UbcH7/UBE2L3 Antibody (24GB1875)[NBP3-51593] -

Validation of UbcH7/UBE2L3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with UbcH7/UBE2L3 antibody (1:2,000) and analyzed using BD flow cytometer.
UbcH7/UBE2L3 Antibody (24GB1875)

Flow Cytometry: UbcH7/UBE2L3 Antibody (24GB1875)[NBP3-51593] -

Flow cytometric analysis of UbcH7/UBE2L3expression in HepG2 cells using UbcH7/UBE2L3 antibody (1:2,000). Green, isotype control; red, UBE2L3.
UbcH7/UBE2L3 Antibody (24GB1875)

Western Blot: UbcH7/UBE2L3 Antibody (24GB1875)[NBP3-51593] -

Western blotting analysis using UbcH7/UBE2L3 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.

Applications for UbcH7/UBE2L3 Antibody (24GB1875)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Gene Symbol

UBE2L3

Additional UbcH7/UBE2L3 Products

Product Documents for UbcH7/UBE2L3 Antibody (24GB1875)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UbcH7/UBE2L3 Antibody (24GB1875)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway
Ubiquitination Cascade Pathway Thumbnail