UBE2I/Ubc9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86887

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBE2I/Ubc9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: IALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for UBE2I/Ubc9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunocytochemistry/ Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunocytochemistry/Immunofluorescence: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody [NBP1-86887] - Staining of human tonsil shows moderate to strong nuclear positivity in non - germinal center cells.
UBE2I/Ubc9 Antibody - BSA Free Western Blot: UBE2I/Ubc9 Antibody - BSA Free [NBP1-86887]

Western Blot: UBE2I/Ubc9 Antibody - BSA Free [NBP1-86887]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
UBE2I/Ubc9 Antibody - BSA Free Western Blot: UBE2I/Ubc9 Antibody - BSA Free [NBP1-86887]

Western Blot: UBE2I/Ubc9 Antibody - BSA Free [NBP1-86887]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
UBE2I/Ubc9 Antibody - BSA Free Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody - BSA Free [NBP1-86887]

Immunohistochemistry-Paraffin: UBE2I/Ubc9 Antibody - BSA Free [NBP1-86887]

Staining of human fallopian tube shows moderate to strong nuclear positivity in glandular cells.

Applications for UBE2I/Ubc9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP1-86887 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2I/Ubc9

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.

Long Name

Ubiquitin-conjugating Enzyme E2I

Alternate Names

p18, SUMO-protein Ligase, Ubc9, UBCE9

Gene Symbol

UBE2I

Additional UBE2I/Ubc9 Products

Product Documents for UBE2I/Ubc9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2I/Ubc9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for UBE2I/Ubc9 Antibody - BSA Free (1)

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  • UBE2I/Ubc9 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 293FT cell lysate and nih3t3 cell lysate
    Species: Human and Mouse
    Verified Customer | Posted 06/29/2017
    UBE2I/Ubc9 Antibody - BSA Free NBP1-86887

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Protocols

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