UBE2J1 Antibody (OTI3D1)

Novus Biologicals | Catalog # NBP2-45402

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3D1
View all UBE2J1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2J1(NP_057105) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2J1 Antibody (OTI3D1)

Western Blot: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Western Blot: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Western Blot: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human prostate tissue.
Flow Cytometry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Flow Cytometry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Flow Cytometry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Jurkat cells, using UBE2J1 antibody,(Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Western Blot: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Western Blot: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2J1.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human colon tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human tonsil tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human lung tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human lymphoma tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Adenocarcinoma of Human breast tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human liver tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human breast tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human pancreas tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Carcinoma of Human lung tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human Kidney tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human prostate tissue.
Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Immunohistochemistry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Human liver tissue.
Flow Cytometry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Flow Cytometry: UBE2J1 Antibody (OTI3D1) [NBP2-45402]

Flow Cytometry: UBE2J1 Antibody (3D1) [NBP2-45402] - Analysis of Hela cells, using UBE2J1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Applications for UBE2J1 Antibody (OTI3D1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2J1

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.

Long Name

Ubiquitin-conjugating Enzyme E2J 1

Alternate Names

CGI-76, HSPC153, NCUBE1, UBC6e

Gene Symbol

UBE2J1

Additional UBE2J1 Products

Product Documents for UBE2J1 Antibody (OTI3D1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2J1 Antibody (OTI3D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for UBE2J1 Antibody (OTI3D1)

There are currently no reviews for this product. Be the first to review UBE2J1 Antibody (OTI3D1) and earn rewards!

Have you used UBE2J1 Antibody (OTI3D1)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail