UBE2K/E2-25K Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16831

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Xenopus (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBE2K/E2-25K Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2K/E2-25K. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2K/E2-25K Antibody - BSA Free

Western Blot: UBE2K/E2-25K Antibody [NBP2-16831]

Western Blot: UBE2K/E2-25K Antibody [NBP2-16831]

Western Blot: UBE2K/E2-25K Antibody [NBP2-16831] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: UBE2K/E2-25K Antibody [NBP2-16831]

Immunocytochemistry/ Immunofluorescence: UBE2K/E2-25K Antibody [NBP2-16831]

Immunocytochemistry/Immunofluorescence: UBE2K/E2-25K Antibody [NBP2-16831] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Western Blot: UBE2K/E2-25K Antibody [NBP2-16831]

Western Blot: UBE2K/E2-25K Antibody [NBP2-16831]

Western Blot: UBE2K/E2-25K Antibody [NBP2-16831] - Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE gel, diluted at 1:1000.

Applications for UBE2K/E2-25K Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2K/E2-25K

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2

Alternate Names

E2-25K, E225K, Hip2, HYPG, LIG, UbcH1, UBE2K, UBE2K-25K

Gene Symbol

UBE2K

UniProt

P61086 (Human)

Additional UBE2K/E2-25K Products

Product Documents for UBE2K/E2-25K Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2K/E2-25K Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail