UBE2M/Ubc12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-55035

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBE2M/Ubc12 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to UBE2M(ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)) The peptide sequence was selected from the N terminal of UBE2M. Peptide sequence IKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISF. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for UBE2M/Ubc12 Antibody - BSA Free

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Human 721_B, Antibody Dilution: 1.0 ug/ml UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.
Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Mouse Brain lysate, Antibody Titration: 0.2-1 ug/ml
Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Sample Type:2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit Secondary Antibody Dilution: 1: 20,000
Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Antibody Titration: 0.2-1 ug/ml Positive control: 721_B cell lysate UBE2M is supported by BioGPS gene expression data to be expressed in 721_B.
Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035]

Western Blot: UBE2M/Ubc12 Antibody [NBP1-55035] - Human MCF7, Antibody Dilution: 1.0 ug/ml UBE2M is supported by BioGPS gene expression data to be expressed in MCF7.

Applications for UBE2M/Ubc12 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBE2M/Ubc12

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.

Alternate Names

UBC-RS2, UBC12, UbcH12

Gene Symbol

UBE2M

UniProt

P61081 (Human)

Additional UBE2M/Ubc12 Products

Product Documents for UBE2M/Ubc12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for UBE2M/Ubc12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail