UBE2R2 Antibody

Novus Biologicals | Catalog # NBP2-15154

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all UBE2R2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBC3B. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2R2 Antibody

Western Blot: UBE2R2 Antibody [NBP2-15154]

Western Blot: UBE2R2 Antibody [NBP2-15154]

Western Blot: UBE2R2 Antibody [NBP2-15154] - Sample (30 ug of whole cell lysate) A: Jurkat 12% SDS PAGE; antibody diluted at 1:5000.
Western Blot: UBE2R2 Antibody [NBP2-15154]

Western Blot: UBE2R2 Antibody [NBP2-15154]

Western Blot: UBE2R2 Antibody [NBP2-15154] - Sample (50 ug of whole cell lysate) A: mouse liver 12% SDS PAGE; antibody diluted at 1:1000.
UBE2R2 Antibody

Immunocytochemistry/ Immunofluorescence: UBE2R2 Antibody [NBP2-15154] -

UBC3B antibody detects UBC3B protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBC3B protein stained by UBC3B antibody (NBP2-15154) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for UBE2R2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2R2

Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq]

Long Name

Ubiquitin-conjugating Enzyme E2R 2

Alternate Names

CDC34B, UBC3B

Gene Symbol

UBE2R2

UniProt

Q712K3 (Human)

Additional UBE2R2 Products

Product Documents for UBE2R2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2R2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail