UBE2T Antibody (OTI2F5)

Name: UBE2T Antibody (OTI2F5)
Brand: Novus Biologicals
SKU: NBP2-02965
Price: 449 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-02965

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # OTI2F5

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Product Specifications

Immunogen

Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

22.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2T Antibody (OTI2F5)

Western Blot: UBE2T Antibody (OTI2F5) [NBP2-02965]

Western Blot: UBE2T Antibody (2F5) [NBP2-02965] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2T (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBE2T.

Immunocytochemistry/Immunofluorescence: UBE2T Antibody (2F5) [NBP2-02965] Staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2T.

Applications for UBE2T Antibody (OTI2F5)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.33 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2T

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM].

Long Name

Ubiquitin-conjugating Enzyme E2T

Alternate Names

HSPC150, PIG50

Entrez Gene IDs

29089 (Human)

Gene Symbol

UBE2T

Additional UBE2T Products

Product Documents for UBE2T Antibody (OTI2F5)

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Certificate of Analysis

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Product Specific Notices for UBE2T Antibody (OTI2F5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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