Ubiquilin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85639

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ubiquilin 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%). Mouse reactivity reported in scientific literature (PMID: 26141599), (PMID: 26152284).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ubiquilin 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ubiquilin 2 Antibody [NBP1-85639]

Immunocytochemistry/ Immunofluorescence: Ubiquilin 2 Antibody [NBP1-85639]

Immunocytochemistry/Immunofluorescence: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cerebellum shows moderate to strong cytoplasmic and nuclear positivity in Purkinje cells.
Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons and glial cells.
Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639]

Immunohistochemistry-Paraffin: Ubiquilin 2 Antibody [NBP1-85639] - Staining of human placenta shows moderate to strong cytoplasmic positivity in cytotrophoblast.
Simple Western: Ubiquilin 2 Antibody [NBP1-85639]

Simple Western: Ubiquilin 2 Antibody [NBP1-85639]

Simple Western: Ubiquilin 2 Antibody [NBP1-85639] - Simple Western lane view shows a specific band for Ubiquilin 2 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: Ubiquilin 2 Antibody [NBP1-85639]

Simple Western: Ubiquilin 2 Antibody [NBP1-85639]

Simple Western: Ubiquilin 2 Antibody [NBP1-85639] - Electropherogram image(s) of corresponding Simple Western lane view. Ubiquilin 2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
Ubiquilin 2 Antibody - BSA Free Western Blot: Ubiquilin 2 Antibody - BSA Free [NBP1-85639]

Western Blot: Ubiquilin 2 Antibody - BSA Free [NBP1-85639]

Analysis in human cell line A-431.

Applications for Ubiquilin 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF reported in the literature (PMID:26152284). ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 72 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ubiquilin 2

Ubiquilin 2 encodes an ubiquitin-like protein (ubiquilin) that shares high degree of similarity with related products in yeast, rat and frog. Ubiquilins contain a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation. This ubiquilin has also been shown to bind the ATPase domain of the Hsp70-like Stch protein.

Alternate Names

Chap1, CHAP1/DSK2, DSK2, DSK2 homolog, FLJ10167, FLJ56541, hPLIC-2, N4BP4LIC-2, Nedd4 binding protein 4, PLIC-2, PLIC2Dsk2, Protein linking IAP with cytoskeleton 2, RIHFB2157, ubiquilin 2, ubiquilin-2, Ubiquitin-like product Chap1/Dsk2

Gene Symbol

UBQLN2

Additional Ubiquilin 2 Products

Product Documents for Ubiquilin 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Ubiquilin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ubiquilin 2 Antibody - BSA Free

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Protocols

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