Ubiquitin C Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94633

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ubiquitin C Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UBC (NP_066289.3). MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ubiquitin C Antibody - BSA Free

Ubiquitin C Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Ubiquitin C Antibody - BSA Free [NBP2-94633] -

Immunocytochemistry/ Immunofluorescence: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunofluorescence analysis of HepG2 cells using Ubiquitin C Rabbit pAb (A3207) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Ubiquitin C Antibody - BSA Free

Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] -

Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded rat large intestine tissue using Ubiquitin C Rabbit pAb (A3207) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Ubiquitin C Antibody - BSA Free

Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] -

Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded human spleen tissue using Ubiquitin C Rabbit pAb (A3207) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Ubiquitin C Antibody - BSA Free

Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] -

Immunohistochemistry: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Immunohistochemistry analysis of Ubiquitin C in paraffin-embedded mouse large intestine tissue using Ubiquitin C Rabbit pAb (A3207) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Ubiquitin C Antibody - BSA Free

Western Blot: Ubiquitin C Antibody - BSA Free [NBP2-94633] -

Western Blot: Ubiquitin C Antibody - BSA Free [NBP2-94633] - Western blot analysis of various lysates using Ubiquitin C Rabbit pAb (A3207) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime: 45s.

Applications for Ubiquitin C Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:100

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ubiquitin C

Ubiquitin is a small protein that is composed of 76 amino acids and its sequence is highly conserved throughout evolution from invertebrates to mammals. In the cytoplasm, ubiquitin is involved in ATP-dependent nonlysomal proteolysis of various proteins. In the nucleus, ubiquitin is conjugated to histone2A and may play a role in regulation of chromatin structure and/or regulation of transcriptional activity.Ubiquitin(76amino acid) was overexpressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

HMG20, PolyUbiquitin, polyubiquitin-B, Polyubiquitin-C, UBC, Ubiquitin-40S ribosomal protein S27a, ubiquitin-60S ribosomal protein L40

Gene Symbol

UBC

Additional Ubiquitin C Products

Product Documents for Ubiquitin C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Ubiquitin C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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