![Western Blot: UBR4 Antibody [NBP1-28724]](https://resources.rndsystems.com/images/products/UBR4-Antibody-Western-Blot-NBP1-28724-img0002.jpg "Western Blot: UBR4 Antibody [NBP1-28724]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 5133 and 5183 of human retinoblastoma-associated factor 600 using the numbering given in entry NP_065816.1 (GeneID 23352).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for UBR4 Antibody
Western Blot: UBR4 Antibody [NBP1-28724]
Western Blot: UBR4 Antibody [NBP1-28724] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.04 ug/ml.![Immunohistochemistry: UBR4 Antibody [NBP1-28724]](https://resources.rndsystems.com/images/products/UBR4-Antibody-Immunohistochemistry-NBP1-28724-img0005.jpg "Immunohistochemistry: UBR4 Antibody [NBP1-28724]")
Immunohistochemistry: UBR4 Antibody [NBP1-28724]
Immunohistochemistry: UBR4 Antibody [NBP1-28724] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- p600 used at a dilution of 1:200 (1ug/ml). Detection: DABApplications for UBR4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100- 1:500
Immunohistochemistry-Paraffin
1:100- 1:500
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: UBR4
Alternate Names
600 kDa retinoblastoma protein-associated factor, EC 6.3.2, EC 6.3.2.-, KIAA0462E3 ubiquitin-protein ligase UBR4, KIAA1307zinc finger, UBR1 type 1, N-recognin-4, p600, RBAF600FLJ41863, Retinoblastoma-associated factor of 600 kDa, ubiquitin protein ligase E3 component n-recognin 4, Zinc finger UBR1-type protein 1, ZUBR1retinoblastoma-associated factor 600-like protein
Entrez Gene IDs
23352 (Human)
Gene Symbol
UBR4
UniProt
Additional UBR4 Products
Product Documents for UBR4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UBR4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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