UBR4 Antibody

Novus Biologicals | Catalog # NBP1-45235

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all UBR4 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-NTTRATSREEKNLQ corresponding to C-Terminus according to NP_065816.2.

Epitope

C-Terminus

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for UBR4 Antibody

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-45235]

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-45235]

Immunohistochemistry-Paraffin: UBR4 Antibody [NBP1-45235] - Staining of Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for UBR4 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

1:10-1:500

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the calculated size of 574 kDa band according to NP_065816.2. IHC-P: Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBR4

p600 is an E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Together with clathrin, p600 forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization. Additionally p600 regulates integrin-mediated signaling and may play a role in activation of FAK in response to cell-matrix interactions.

Alternate Names

600 kDa retinoblastoma protein-associated factor, EC 6.3.2, EC 6.3.2.-, KIAA0462E3 ubiquitin-protein ligase UBR4, KIAA1307zinc finger, UBR1 type 1, N-recognin-4, p600, RBAF600FLJ41863, Retinoblastoma-associated factor of 600 kDa, ubiquitin protein ligase E3 component n-recognin 4, Zinc finger UBR1-type protein 1, ZUBR1retinoblastoma-associated factor 600-like protein

Entrez Gene IDs

23352 (Human); 69116 (Mouse); 313658 (Rat)

Gene Symbol

UBR4

UniProt

NP_065816.2 (Human)

Additional UBR4 Products

Product Documents for UBR4 Antibody

Certificate of Analysis

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Product Specific Notices for UBR4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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