UBR5/EDD Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12948

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Mouse (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBR5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human UBR5/EDD. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

309 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for UBR5/EDD Antibody - BSA Free

Western Blot: UBR5/EDD Antibody [NBP3-12948]

Western Blot: UBR5/EDD Antibody [NBP3-12948]

Western Blot: UBR5/EDD Antibody [NBP3-12948] - U87-MG whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with UBR5/EDD antibody (NBP3-12948) diluted at 1:500.
UBR5/EDD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: UBR5/EDD Antibody [NBP3-12948] -

EDD antibody detects EDD protein at nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EDD protein stained by EDD antibody (NBP3-12948) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for UBR5/EDD Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UBR5

EDD encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Long Name

Ubiquitin Protein Ligase E3 Component N-recognin 5

Alternate Names

DD5, EDD, EDD1, HYD

Gene Symbol

UBR5

Additional UBR5 Products

Product Documents for UBR5/EDD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for UBR5/EDD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases

Customer Reviews for UBR5/EDD Antibody - BSA Free

There are currently no reviews for this product. Be the first to review UBR5/EDD Antibody - BSA Free and earn rewards!

Have you used UBR5/EDD Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies