UCH-L1/PGP9.5 Antibody (SPM574)

Novus Biologicals | Catalog # NBP2-32895

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM574
View all UCH-L1/PGP9.5 Primary Antibodies »

Product Specifications

Immunogen

UCH-L1/PGP9.5 protein from brain (Uniprot: P09936)

Localization

Cytoplasmic. Endoplasmic Reticulum membrane

Marker

pan-Neuronal Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34807)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for UCH-L1/PGP9.5 Antibody (SPM574)

Western Blot: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895]

Western Blot: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895]

Western Blot: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Western Blot Analysis of human Brain lysate using UCH-L1/PGP9.5 Antibody (SPM574)
Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895]

Immunocytochemistry/ Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895]

Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (SPM574) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)
Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895]

Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Formalin-fixed. paraffin-embedded rat cerebellum stained with Pgp9.5/UchL1 Monoclonal antibody (SPM574)

Applications for UCH-L1/PGP9.5 Antibody (SPM574)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: UCH-L1/PGP9.5

Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) is a soluble cytoplasmic protein found in neurons and in cells of the diffuse neuroendocrine system, and their respective tumors. Therefore, UCH-L1 has been widely used as a peripheral nerve fiber marker. UCHL-1 functions as a tissue-specific ubiquitin carboxyl terminal hydrolase isoenzyme involved in the processing of both ubiquitin precursors and ubiquitinated proteins.

UCH L1 is down-regulated in brains from Parkinson disease and Alzheimer disease patients, and certian site specific mutations in the UCHL1 gene can either increase or decrese the risk of Parkinson's and/or Alzheimer's neurodegenerative diseases.

Human UCHL 1 and the closely related UCHL3 protein have one of the most complicated knot structures ever discovered, with five knot crossings. This knot structure is expected to help the protein resist degradation in the proteasome.

Long Name

Ubiquitin C-terminal Hydrolase L1

Alternate Names

PARK5, PGP9.5, UCHL1

Gene Symbol

UCHL1

UniProt

P09936 (Human)

Additional UCH-L1/PGP9.5 Products

Product Documents for UCH-L1/PGP9.5 Antibody (SPM574)

Certificate of Analysis

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Product Specific Notices for UCH-L1/PGP9.5 Antibody (SPM574)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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