UCH-L3 Antibody (007) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-90878
Recombinant Monoclonal Antibody
![Western Blot: UCH-L3 Antibody (007) [NBP2-90878]](https://resources.rndsystems.com/images/products/UCH-L3-Antibody-007-Western-Blot-NBP2-90878-img0004.jpg "Western Blot: UCH-L3 Antibody (007) [NBP2-90878]")
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 007 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Rat UCH-L3 (Uniprot#: Q91Y78; Glu2-Ala230).
Specificity
Has cross-reactivity in ELISA with: Human UCHL3Mouse UCHL3
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for UCH-L3 Antibody (007) - Azide and BSA Free
Western Blot: UCH-L3 Antibody (007) [NBP2-90878]
Western Blot: UCH-L3 Antibody (007) [NBP2-90878] - Anti-UCHL3 rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:26 kDa. Observed band size:31 kDa.![Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878]](https://resources.rndsystems.com/images/products/UCH-L3-Antibody-007-Immunohistochemistry-Paraffin-NBP2-90878-img0003.jpg "Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878]")
Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878]
Immunohistochemistry-Paraffin: UCH-L3 Antibody (007) [NBP2-90878] - Immunochemical staining of rat UCHL3 in rat testis with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).![Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878]](https://resources.rndsystems.com/images/products/UCH-L3-Antibody-007-Immunoprecipitation-NBP2-90878-img0005.jpg "Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878]")
Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878]
Immunoprecipitation: UCH-L3 Antibody (007) [NBP2-90878] - rat UCHL3 was immunoprecipitated using: Lane A:0.5 mg k562 Whole Cell Lysate. Lane B:0.5 mg Jurkat Whole Cell Lysate. Lane C:0.5 mg SH-SY5Y Whole Cell Lysate. 2 uL anti-rat UCHL3 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-rat UCHL3 rabbit monoclonal antibody,at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 26 kDa.Applications for UCH-L3 Antibody (007) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UCH-L3
Long Name
Ubiquitin C-terminal Hydrolase L3
Alternate Names
Ubiquitin Thioesterase L3, UCHL3
Gene Symbol
UCHL3
Additional UCH-L3 Products
Product Documents for UCH-L3 Antibody (007) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UCH-L3 Antibody (007) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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