UCP2 Antibody (3F1B9) - BSA Free

Novus Biologicals | Catalog # NBP2-52582

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 3F1B9

Format

BSA Free
View all UCP2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human UCP2 (AA: 1-309) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse UCP2 Antibody (3F1B9) - BSA Free (NBP2-52582) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for UCP2 Antibody (3F1B9) - BSA Free

Western Blot: UCP2 Antibody (3F1B9)BSA Free [NBP2-52582]

Western Blot: UCP2 Antibody (3F1B9)BSA Free [NBP2-52582]

Western Blot: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis using UCP2 mAb against human UCP2 (AA: 1-309) recombinant protein. (Expected MW is 36.1 kDa)
Immunocytochemistry/ Immunofluorescence: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Immunocytochemistry/ Immunofluorescence: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Immunocytochemistry/Immunofluorescence: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis of Hela cells using UCP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow Cytometry: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Flow Cytometry: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

Flow Cytometry: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis of Hela cells using UCP2 mouse mAb (green) and negative control (red).
Western Blot: UCP2 Antibody (3F1B9)BSA Free [NBP2-52582]

Western Blot: UCP2 Antibody (3F1B9)BSA Free [NBP2-52582]

Western Blot: UCP2 Antibody (3F1B9) [NBP2-52582] - Analysis using UCP2 mAb against HEK293 (1) and UCP2 hIgGFc transfected HEK293 (2) cell lysate.
ELISA: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

ELISA: UCP2 Antibody (3F1B9) - BSA Free [NBP2-52582]

ELISA: UCP2 Antibody (3F1B9) [NBP2-52582] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Applications for UCP2 Antibody (3F1B9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: UCP2

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.

Long Name

Uncoupling Protein 2

Alternate Names

SLC25A8, UCPH

Gene Symbol

UCP2

Additional UCP2 Products

Product Documents for UCP2 Antibody (3F1B9) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UCP2 Antibody (3F1B9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for UCP2 Antibody (3F1B9) - BSA Free

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    • Q: Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
      A: Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.
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