ULK3 Antibody (5Z7Z6)

Novus Biologicals | Catalog # NBP3-16622

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Z7Z6 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 373-472 of human ULK3 (Q6PHR2). KPRLLAALEVASAAMAKEEAAGGEQDALDLYQHSLGELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRESRWEADTLDKEGLSESVRSSCTLQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ULK3 Antibody (5Z7Z6)

Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622]

Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622]

Immunocytochemistry/Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Immunofluorescence analysis of C6 cells using ULK3 Rabbit mAb (NBP3-16622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622]

Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622]

Immunocytochemistry/Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Immunofluorescence analysis of NIH-3T3 cells using ULK3 Rabbit mAb (NBP3-16622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ULK3 Antibody (5Z7Z6)

Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622] -

Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Confocal imaging of C2C12 cells using ULK3 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ULK3 Antibody (5Z7Z6)

Western Blot: ULK3 Antibody (5Z7Z6) [NBP3-16622] -

Western Blot: ULK3 Antibody (5Z7Z6) [NBP3-16622] - Western blot analysis of lysates from 293T cells using ULK3 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 10s.

Applications for ULK3 Antibody (5Z7Z6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ULK3

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Alternate Names

DKFZp434C131, EC 2.7.11.1, FLJ90566, serine/threonine-protein kinase ULK3, Unc-51-like kinase 3, unc-51-like kinase 3 (C. elegans)

Gene Symbol

ULK3

Additional ULK3 Products

Product Documents for ULK3 Antibody (5Z7Z6)

Certificate of Analysis

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Product Specific Notices for ULK3 Antibody (5Z7Z6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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