UQCRC1 Antibody (1311) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43596
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Primate (100%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1311
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%), Bovine (88%).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for UQCRC1 Antibody (1311) - Azide and BSA Free
Western Blot: UQCRC1 Antibody (1311) [NBP2-43596]
Western Blot: UQCRC1 Antibody (1311) [NBP2-43596] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with UQCRC1 (1311) antibody diluted at 1:1000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Western Blot: UQCRC1 Antibody (1311) [NBP2-43596]
Western Blot: UQCRC1 Antibody (1311) [NBP2-43596] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE UQCRC1 antibody [1311] dilution: 1:1000Western Blot: UQCRC1 Antibody (1311) [NBP2-43596]
Western Blot: UQCRC1 Antibody (1311) [NBP2-43596] - Analysis of A. 50 ug mouse brain lysate/extract 10 % SDS-PAGE UQCRC1 antibody [1311] dilution: 1:1000Western Blot: UQCRC1 Antibody (1311) [NBP2-43596]
Western Blot: UQCRC1 Antibody (1311) [NBP2-43596] - Analysis of A. 50 ug rat brain lysate/extract 10 % SDS-PAGE UQCRC1 antibody [1311] dilution: 1:1000Immunocytochemistry/ Immunofluorescence: UQCRC1 Antibody (1311) [NBP2-43596] -
UQCRC1 antibody [GT1311] detects UQCRC1 protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 5 min.
Green: UQCRC1 protein stained by UQCRC1 antibody [GT1311] (NBP2-43596) diluted at 1:500.
Blue: Hoechst 33342 staining.
Applications for UQCRC1 Antibody (1311) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: UQCRC1
Alternate Names
Complex III subunit 1, Core protein I, cytochrome b-c1 complex subunit 1, mitochondrial, D3S3191, QCR1, ubiquinol-cytochrome c reductase core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCR1
Gene Symbol
UQCRC1
Additional UQCRC1 Products
Product Documents for UQCRC1 Antibody (1311) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UQCRC1 Antibody (1311) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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