UQCRC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20815

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Monkey (97%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UQCRC1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.

Reactivity Notes

Mouse (88%), Rat (87%), Rhesus Monkey (97%), Chimpanzee (100%), Bovine (88%).

Localization

Mitochondrion inner membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UQCRC1 Antibody - BSA Free

UQCRC1 Antibody - BSA Free

Western Blot: UQCRC1 Antibody [NBP2-20815] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody (NBP2-20815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
UQCRC1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: UQCRC1 Antibody [NBP2-20815] -

NYREN18 antibody [N1N3] detects NYREN18 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min.
Green: NYREN18 protein stained by NYREN18 antibody [N1N3] (NBP2-20815) diluted at 1:500.
Blue: Hoechst 33342 staining.
UQCRC1 Antibody - BSA Free

Western Blot: UQCRC1 Antibody [NBP2-20815] -

UQCRC1 antibody detects UQCRC1 protein by western blot analysis.
A Huh7 whole cell lysate/extract
B Hep3B whole cell lysate/extract
C HepG2 whole cell lysate/extract
10% SDS-PAGE
UQCRC1 antibody (NBP2-20815) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
UQCRC1 Antibody - BSA Free

Western Blot: UQCRC1 Antibody [NBP2-20815] -

UQCRC1 antibody detects UQCRC1 protein by western blot analysis.
A. 30 ug 293T whole cell lysate/extract
B. 30 ug A431 whole cell lysate/extract
C. 30 ug HeLa whole cell lysate/extract
D. 30 ug HepG2 whole cell lysate/extract
E. 30 ug A375 whole cell lysate/extract
10% SDS-PAGE
UQCRC1 antibody (NBP2-20815) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for UQCRC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:1000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UQCRC1

This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.

Alternate Names

Complex III subunit 1, Core protein I, cytochrome b-c1 complex subunit 1, mitochondrial, D3S3191, QCR1, ubiquinol-cytochrome c reductase core protein I, Ubiquinol-cytochrome-c reductase complex core protein 1, UQCR1

Gene Symbol

UQCRC1

UniProt

P31930 (Human)

Additional UQCRC1 Products

Product Documents for UQCRC1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UQCRC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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