UQCRC2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-80861
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0012.jpg "Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]")
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Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Western Blot, Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: PVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for UQCRC2 Antibody - BSA Free
![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0013.jpg "Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]")
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human cerebral cortex, heart muscle, liver and pancreas using Anti-UQCRC2 antibody NBP1-80861 (A) shows similar protein distribution across tissues to independent antibody NBP1-80862 (B).![Western Blot: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Western-Blot-NBP1-80861-img0014.jpg "Western Blot: UQCRC2 Antibody [NBP1-80861]")
![Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80861-img0009.jpg "Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody [NBP1-80861]")
Immunocytochemistry/ Immunofluorescence: UQCRC2 Antibody [NBP1-80861]
Immunocytochemistry/Immunofluorescence: UQCRC2 Antibody [NBP1-80861] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0016.jpg "Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]")
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human cerebral cortex.![Western Blot: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Western-Blot-NBP1-80861-img0008.jpg "Western Blot: UQCRC2 Antibody [NBP1-80861]")
Western Blot: UQCRC2 Antibody [NBP1-80861]
Western Blot: UQCRC2 Antibody [NBP1-80861] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0010.jpg "Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]")
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human heart muscle shows high expression.![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0011.jpg "Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]")
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human pancreas shows low expression as expected.![Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]](https://resources.rndsystems.com/images/products/UQCRC2-Antibody-Immunohistochemistry-Paraffin-NBP1-80861-img0015.jpg "Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]")
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861]
Immunohistochemistry-Paraffin: UQCRC2 Antibody [NBP1-80861] - Staining of human liver.Applications for UQCRC2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation
See Simple Western Antibody Database for Simple Western validation
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: UQCRC2
Alternate Names
Complex III subunit 2, Core protein II, cytochrome b-c1 complex subunit 2, mitochondrial, QCR2, ubiquinol-cytochrome c reductase core protein II, Ubiquinol-cytochrome-c reductase complex core protein 2, UQCR2
Entrez Gene IDs
7385 (Human)
Gene Symbol
UQCRC2
UniProt
Additional UQCRC2 Products
Product Documents for UQCRC2 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for UQCRC2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for UQCRC2 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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