Uromodulin Antibody - Azide and BSA Free

Novus Biologicals | Catalog # H00007369-B01P

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

UMOD (AAH35975, 1 a.a. - 611 a.a.) full-length human protein. MGQPSLTWMLMVVVASWFITTAATDTSEARWCSECHSNATCTEDEAVTTCTCQEGFTGDGLTCVDLDECAIPGAHNCSANSSCVNTPGSFSCVCPEGFRLSPGLGCTDVDECAEPGLSHCHALATCVNVVGSYLCVCPAGYRGDGWHCECSPGSCGPGLDCVPEGDALVCADPCQAHRTLDEYWRSTEYGEGYACDTDLRGWYRPHPSSDEGIVSRKACAHWSGHCCLWDASVQVKACAGGYYVYNLTAPPECHLAYCTDPSSVEGTCEECSIDEDCKSNNGRWHCQCKQDFNITDISLLEHRLECGANDMKVSLGKCQLKSLGFDKVFMYLSDSRCSGFNDRDNRDWVSVVTPARDGPCGTVLTRNETHATYSNTLYLADEIIIRDLNIKINFACSYPLDMKVSLKTALQPMVSALNIRVGGTGMFTVRMALFQTPSYTQPYQGSSVTLSTEAFLYVGTMLDGGDLSRFALLMTNCYATPSSNATDPLKYFIIQDRCPHTRDSTIQVVENGESSQGRFSVQMFRFAGNYDLVYLHCEVYLCDTMNEKCKPTCSGTRFRSGSVIDQSRVLNLGPITRKGVQATVSRAFSSLGLLKVWLPLLLSATLTLTFQ

Specificity

UMOD - uromodulin (uromucoid, Tamm-Horsfall glycoprotein),

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for Uromodulin Antibody - Azide and BSA Free

Western Blot: Uromodulin Antibody [H00007369-B01P]

Western Blot: Uromodulin Antibody [H00007369-B01P]

Western Blot: Uromodulin Antibody [H00007369-B01P] - Analysis of UMOD expression in transfected 293T cell line by UMOD polyclonal antibody. Lane 1: UMOD transfected lysate(67.21 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Uromodulin Antibody [H00007369-B01P]

Immunocytochemistry/ Immunofluorescence: Uromodulin Antibody [H00007369-B01P]

Immunocytochemistry/Immunofluorescence: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: Uromodulin Antibody [H00007369-B01P]

Immunohistochemistry-Paraffin: Uromodulin Antibody [H00007369-B01P]

Immunohistochemistry-Paraffin: Uromodulin Antibody [H00007369-B01P] - Analysis of purified antibody to UMOD on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)

Applications for Uromodulin Antibody - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. May also be used for IF, IHC-P. Use in FLOW reported in scientific literature (Santelli A et al).

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Uromodulin

This gene encodes uromodulin, the most abundant protein in normal urine. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. Uromodulin may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of uromodulin in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the autosomal dominant renal disorders medullary cystic kidney disease-2 (MCKD2) and familial juvenile hyperuricemic nephropathy (FJHN). These disorders are characterized by juvenile onset of hyperuricemia, gout, and progressive renal failure. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq]

Alternate Names

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, Uromucoid

Entrez Gene IDs

7369 (Human)

Gene Symbol

UMOD

Additional Uromodulin Products

Product Documents for Uromodulin Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Uromodulin Antibody - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Uromodulin Antibody - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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