Uroplakin Ia Antibody (UPK1A/2921)

Novus Biologicals | Catalog # NBP2-79733

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Key Product Details

Species Reactivity

Human, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # UPK1A/2921
View all Uroplakin Ia Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 114-173) of human Uroplakin Ia (UPK1A) protein (exact sequence is proprietary) (Uniprot: O00322)

Reactivity Notes

Canine reactivity reported from a verified customer review.

Localization

Cell Surface

Marker

Urothelial Differentiation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79868)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Uroplakin Ia Antibody (UPK1A/2921)

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of canine bladder tissue using Uroplakin Ia antibody. Uroplakin Ia antibody used at 1:500 concentration in 3% BSA and left at 4C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9. Image from verified customer review.
Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).
Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urinary Bladder stained with Uroplakin Ia Antibody (UPK1A/2921).
Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Immunohistochemistry-Paraffin: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Formalin-fixed, paraffin-embedded human Urothelial Carcinoma stained with Uroplakin Ia Antibody (UPK1A/2921).
Protein Array: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Protein Array: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733]

Protein Array: Uroplakin Ia Antibody (UPK1A/2921) [NBP2-79733] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Uroplakin Ia Antibody (UPK1A/2921) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Uroplakin Ia Antibody (UPK1A/2921)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-79733 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Uroplakin Ia

Alternate Names

TSPAN21, UP1A, UPIA, UPK1A, UPKA

Gene Symbol

UPK1A

UniProt

O00322 (Human)

Additional Uroplakin Ia Products

Product Documents for Uroplakin Ia Antibody (UPK1A/2921)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Uroplakin Ia Antibody (UPK1A/2921)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Integrins and Associated Molecules

Citations for Uroplakin Ia Antibody (UPK1A/2921)

Customer Reviews for Uroplakin Ia Antibody (UPK1A/2921) (1)

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Customer Images

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  • Uroplakin Ia Antibody (UPK1A/2921)
    Name: Anonymous
    Application: Affinity Purification
    Sample Tested: Bladder tissue
    Species: Canine
    Verified Customer | Posted 03/08/2022
    UPK1A used at 1:500 concentration in 3%BSA and left at 4 C overnight on paraffin embedded canine bladder tissue. HIER was performed in Tris/EDTA buffer, pH 9.
    Uroplakin Ia Antibody (UPK1A/2921) NBP2-79733
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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