Uroplakin III Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86874

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QILNAYLVRVGANGTCLWDPNFQGLCNPPLSAATEYRFKYVLVNMSTGLVEDQTLWSDPIRTNQLTPYSTIDTWPGRRSGGMIVIT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Uroplakin III Antibody - BSA Free

Uroplakin III Antibody - BSA Free Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Uroplakin III Antibody - BSA Free Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Staining of human liver shows no positivity in hepatocytes as expected.
Uroplakin III Antibody - BSA Free Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Uroplakin III Antibody - BSA Free Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Staining of human stomach shows no positivity in glandular cells as expected.
Uroplakin III Antibody - BSA Free Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Staining of human liver, pancreas, stomach and urinary bladder using Anti-UPK3A antibody NBP1-86874 (A) shows similar protein distribution across tissues to independent antibody NBP1-86873 (B).
Uroplakin III Antibody - BSA Free Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Immunohistochemistry-Paraffin: Uroplakin III Antibody - BSA Free [NBP1-86874]

Analysis in human urinary bladder and pancreas tissues Corresponding UPK3A RNA-seq data are presented for the same tissues.

Applications for Uroplakin III Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Uroplakin III

Uroplakins are specific differentiation products of terminally differentiated superficial urothelial cells. Uroplakin III is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas.

Alternate Names

MGC119178, UP3a, UPIII, UPIIIA, UPK3, UPK3A, uroplakin 3, uroplakin 3A, Uroplakin III, uroplakin3a, uroplakin-3a

Gene Symbol

UPK3A

Additional Uroplakin III Products

Product Documents for Uroplakin III Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Uroplakin III Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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