Use1/UBE2Z Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-97553

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Use1/UBE2Z Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Use1/UBE2Z fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Use1/UBE2Z Antibody - BSA Free

Western Blot: Use1/UBE2Z Antibody [NBP2-97553]

Western Blot: Use1/UBE2Z Antibody [NBP2-97553]

Western Blot: Use1/UBE2Z Antibody [NBP2-97553] - Anti-Use1/UBE2Z rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 33 kDa
Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP2-97553]

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP2-97553]

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: Use1/UBE2Z Antibody [NBP2-97553]

Immunoprecipitation: Use1/UBE2Z Antibody [NBP2-97553]

Immunoprecipitation: Use1/UBE2Z Antibody [NBP2-97553] - Use1/UBE2Z was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-Use1/UBE2Z rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Use1/UBE2Z rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 29 kDa
Use1/UBE2Z Antibody - BSA Free

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody - BSA Free [NBP2-97553] -

Immunohistochemistry-Paraffin: Use1/UBE2Z Antibody - BSA Free [NBP2-97553] - Immunochemical staining of human Use1/UBE2Z in human duodenum with NBP2-97553 at 1:100 dilution, formalin-fixed paraffin embedded sections.
Use1/UBE2Z Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Use1/UBE2Z Antibody [NBP2-97553] -

Immunocytochemistry/Immunofluorescence: Use1/UBE2Z Antibody [NBP2-97553] - Immunofluorescence staining of Use1/UBE2Z in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97553 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for Use1/UBE2Z Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Use1/UBE2Z

SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER

Alternate Names

UBE2Z

Gene Symbol

USE1

Additional Use1/UBE2Z Products

Product Documents for Use1/UBE2Z Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Use1/UBE2Z Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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