Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all USP1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 450 and 500 of human ubiquitin-specific-processing protease 1 using the numbering given in entry NP_003359.3 (GeneID 7398).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for USP1 Antibody - BSA Free

Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117]

Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117]

Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- USP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: USP1 Antibody [NB100-88117]

Immunoprecipitation: USP1 Antibody [NB100-88117]

Immunoprecipitation: USP1 Antibody [NB100-88117] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88117 used for IP at 3 mcg/mg lysate. USP1 was also immunoprecipitated by NB100-88116 and NB100-88118, which recognize other epitopes.
Immunoprecipitation: USP1 Antibody [NB100-88117]

Immunoprecipitation: USP1 Antibody [NB100-88117]

Immunoprecipitation: USP1 Antibody [NB100-88117] - Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody NB100-88117 used for IP at 6 ug per reaction. USP1 was also immunoprecipitated by rabbit anti-USP1 antibodies NB100-88116 and NB100-88118. For blotting immunoprecipitated USP1, NB100-88117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for USP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000- 1:5000

Immunohistochemistry-Paraffin

1:1000- 1:5000

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 or TrisEDTA pH 9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: USP1

This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]

Long Name

Ubiquitin Specific Protease 1

Alternate Names

Deubiquitinating Enzyme 1, Ubiquitin Carboxyl-Terminal Hydrolase 1, Ubiquitin Thioesterase 1, UBP

Entrez Gene IDs

7398 (Human)

Gene Symbol

USP1

UniProt

NP_003359.3 (Human)

Additional USP1 Products

Product Documents for USP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for USP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Deubiquitinating Enzymes (DUBs)

Citations for USP1 Antibody - BSA Free

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Protocols

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FAQs

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